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Human skin is highly susceptible to injuries from routine activities such as accidents and illnesses, thus requiring effective wound-healing treatment. The wound healing process can be significantly prolonged due to bacterial infections such as Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa). The introduction of nanoparticles (NPs) has shown a promising advancement in wound care management. This study explored the combination effects of greenly synthesized zinc oxide (ZnO) nanoparticles using Aloe Vulgarize (AV) leaf extract with chitosan (CS) nanoparticles (ZnO/AV-CS NPs) on antibacterial, molecular docking, and wound healing. The chemical composition and morphology of the NPs were characterized using Fourier Transform Infrared spectroscopy (FTIR) and Field Emission Scanning Electron Microscope (FESEM), respectively. Comparative analyses were conducted with a previously synthesized compound (ZnO NPs/AV (probe)) and a commercial product, which is Zn-hyaluronate (CURIOSIN™). The study successfully synthesized ZnO/AV-CS NPs with spherical shape morphology, thus demonstrating the ability to inhibit Methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli) through the disc diffusion method, evidenced by a zone of inhibition (ZOI) of 14.0 mm and 22.0 mm, respectively. The molecular docking revealed that ZnO/AV-CS NPs exhibited larger binding affinity with binding energies of −11.0 kcal/mol and − 17.2 kcal/mol on 1FCM and 1MWU proteins from E. coli and MRSA, respectively, relative to ZnO NPs/AV. Additionally, molecular docking highlighted the significant role of hydrogen bonds in bacterial growth inhibition. In the scratch assay, the ZnO/AV-CS NPs achieved a wound closure rate of 73.9% in HDF cells, surpassing the performance of the commercial products at 61.1% of wound healing. In conclusion, ZnO/AV-CS NPs exhibited superior wound healing potential with significant antibacterial effects compared to commercial Zn-hyaluronate (CURIOSIN™) by demonstrating rapid wound closure, attributed to their effective inhibition on bacterial colonization at the wound site, which is beneficial in wound care therapy.

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Combine Effects of Zinc Oxide and Chitosan Nanoparticles: Antibacterial, Molecular Docking and Wound Healing Analyses

  • 会议论文

  • 发表日期：

    2026年

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  • Nur Syafiqah Farhanah Dzulkharnien,
  • Rosiah Rohani,
  • Noorhisham Tan Kofli,
  • Noor Alicezah Mohd Kasim,
  • Melonney Patrick,
  • Suhaila Abdul Muid

摘要

Human skin is highly susceptible to injuries from routine activities such as accidents and illnesses, thus requiring effective wound-healing treatment. The wound healing process can be significantly prolonged due to bacterial infections such as Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa). The introduction of nanoparticles (NPs) has shown a promising advancement in wound care management. This study explored the combination effects of greenly synthesized zinc oxide (ZnO) nanoparticles using Aloe Vulgarize (AV) leaf extract with chitosan (CS) nanoparticles (ZnO/AV-CS NPs) on antibacterial, molecular docking, and wound healing. The chemical composition and morphology of the NPs were characterized using Fourier Transform Infrared spectroscopy (FTIR) and Field Emission Scanning Electron Microscope (FESEM), respectively. Comparative analyses were conducted with a previously synthesized compound (ZnO NPs/AV (probe)) and a commercial product, which is Zn-hyaluronate (CURIOSIN™). The study successfully synthesized ZnO/AV-CS NPs with spherical shape morphology, thus demonstrating the ability to inhibit Methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli) through the disc diffusion method, evidenced by a zone of inhibition (ZOI) of 14.0 mm and 22.0 mm, respectively. The molecular docking revealed that ZnO/AV-CS NPs exhibited larger binding affinity with binding energies of −11.0 kcal/mol and − 17.2 kcal/mol on 1FCM and 1MWU proteins from E. coli and MRSA, respectively, relative to ZnO NPs/AV. Additionally, molecular docking highlighted the significant role of hydrogen bonds in bacterial growth inhibition. In the scratch assay, the ZnO/AV-CS NPs achieved a wound closure rate of 73.9% in HDF cells, surpassing the performance of the commercial products at 61.1% of wound healing. In conclusion, ZnO/AV-CS NPs exhibited superior wound healing potential with significant antibacterial effects compared to commercial Zn-hyaluronate (CURIOSIN™) by demonstrating rapid wound closure, attributed to their effective inhibition on bacterial colonization at the wound site, which is beneficial in wound care therapy.