Extraction, Optimization and Phytochemical Screening of Bulbine frutescens and Cotyledon orbiculata Leaves
摘要
Optimization of extraction parameters is crucial for maximizing the yield of the phytochemical content. The aim of this study was to optimize the extraction parameters for Bulbine frutescens (BF) and Cotyledon orbiculata (CO) leaves, which are known for their wound healing and anti-inflammation. The extraction of BF with methanol and ethanol at room temperature achieved the highest extract yields of 8.48 and 9.03%, respectively. Methanol and ethanol extractions of CO resulted in extract yields of 14.02 and 10.52%, respectively. The extraction with ethyl acetate at the ratio of 1:20 presented the highest extract yield of 15.08% while the extraction at 1:10 achieved extract yield of 5.47%. Extractions of BF with water, methanol and ethanol at 40 °C resulted in the extract yields of 31.80, 46.25 and 61.99%, respectively. Methanol extraction of CO achieved the highest extract yield of 16.96%. Phytochemical screening of the BF extracts revealed the presence of flavonoids in all the extracts, while flavonoids were only present in the CO water, dichloromethane and ethyl acetate extracts. Glycosides were present in all the extracts of BF except dichloromethane, chloroform and hexane extracts. No glycosides were present in all CO extracts. Phenols/Tannins were not present in all BF extracts, while their presence was seen in acetone, dichloromethane, chloroform and ethyl acetate extracts of CO. Presence of saponins was observed in water, acetone, dichloromethane and hexane extracts of BF, but only in the hexane extract of CO. Terpenoids were only observed in the methanol, ethanol, acetone, chloroform and ethyl acetate extracts of BF.