Digitalization is reshaping the concept of entrepreneurship as a driving force in societal transformation. Digital entrepreneurship offers an innovative field that provides equal opportunities, particularly for women, by enabling low-cost start-ups and flexible working conditions independent of time and location. In this context, the relationship between digital entrepreneurship and gender equality has increasingly become a subject of scholarly investigation. This study examines the digital entrepreneurial tendencies of Turkish and Indonesian Generation Z university students representing developing countries. It evaluates the differences in students’ digital entrepreneurial goals based on gender, national background, and whether they received entrepreneurship education. Using a quantitative research design, data were collected through an online survey of 385 undergraduate students at Kastamonu University. The findings reveal that male students exhibit higher levels of digital entrepreneurial intention than female students. Moreover, significant differences were observed between Turkish and Indonesian students regarding their digital entrepreneurial goals. However, taking an entrepreneurship course did not produce a statistically significant difference in students’ digital entrepreneurial tendencies. This result suggests that entrepreneurship education should be supported with content that enhances digital competencies and emphasizes practical application. The study also discusses the influence of cultural values and uncertainty avoidance levels on digital entrepreneurial goals within the framework of Hofstede’s cultural dimensions theory. It provides educational and policy recommendations to increase women’s participation in digital entrepreneurship in developing countries. In this regard, the study offers an original contribution to the digital entrepreneurship literature by shedding light on the interplay between gender equality, cultural differences, and entrepreneurship education.