Surfactant, an acronym of “SURFace ACTive AgeNT”, typically comprises hydrophilic head and hydrophobic tail that help to form a variety of aggregates beyond a threshold concentration. Surfactant gels are formed when surfactant molecules self-assemble into a three-dimensional network structure in a solvent, resulting in the entrapment of solvent molecules within the gel matrices. Formation of surfactant gels are influenced by different factors, viz., surfactant concentration, temperature, pH, and the presence of additives (like polymers, co-solvents, electrolytes, etc.). Formulation of surfactant gels involve the preparation of surfactant solutions followed by inducing gelation through methods, viz., temperature variation, pH adjustment, salinity or addition of co-solvents. Commonly used surfactants for gel formation include anionic, cationic, non-ionic, and zwitterionic surfactants, each imparting unique property to the resulting gel. Characterization of surfactant gel is crucial for understanding their structure–property relationships and optimizing their performances. Rheology, microscopy, UV–visible absorption and emission spectroscopy, and thermal analysis are usually employed to evaluate parameters like gel strength, microstructure, viscoelastic behaviour, and thermal stability. Application of surfactant gels span a wide range that include pharmaceuticals, cosmetics, personal care products, agriculture, and environmental remediation. In cosmetics and personal care products, they serve as thickeners, emulsifiers, and stabilizers in formulations like creams, lotions, and gels. In agriculture, surfactant gels are employed for soil stabilization, water retention, and pesticide delivery. Additionally, surfactant gels find use in environmental applications, viz., oil spill clean-up and groundwater remediation, owing to their ability to encapsulate and remove contaminants. The present chapter emphasizes the importance of surfactant-based gels in the topical delivery of drugs. These surfactant based gel formulations are useful in skin debridement as hydrophobic regions of the preformed micelles can trap the necrotic parts of tissues infected with microbes and the hydrophilic regions allow it to be washed away. The main use for such combination of surfactants include antimicrobial and antibiotic carriers as well as cleaning solutions and wound washes. This chapter explores the multifaceted role of surfactants in wound care, emphasizing their dual function as cleansing agents and carriers for antimicrobial agents through comprehensive examinations of the applications and advancements in surfactant-based wound care. Further research and development efforts in surfactant gel technology hold great potential for addressing current challenges and driving innovation in various industries.