Wounding stress in plants, caused by mechanical injury, herbivory, or environmental disturbances, triggers complex physiological and biochemical responses aimed at restoring cellular integrity and preventing pathogen invasion. Gamma-aminobutyric acid (GABA) mitigates the adverse effects of wounding stress because of its multifaceted role in plant defense, signaling cascades, ion flux regulation, reactive oxygen species (ROS) scavenging, and modulation of metabolic pathways. Upon wounding, GABA rapidly accumulates at the injury site, facilitating early signaling responses and enhancing tissue repair. GABA also influences the expression of wound stress-responsive genes and interacts with key phytohormones such as jasmonate (JA) and salicylic acid (SA) to orchestrate an integrated defense response. Overall, the protective role of GABA against wounding stress underscores its significance as a target for enhancing plant defense mechanisms in agricultural systems challenged by mechanical injuries and pest attacks, along with the restoration of crop growth and productivity.