Soil microbiomes are crucial in supporting plant development and maintaining soil functions for sustainable crop production. Soil functions improve through complex interactions between biochar and microbiome. Biochar, a carbon-rich substance derived from biomass pyrolysis, has emerged as a multifunctional soil amendment with significant potential to improve soil functions. Acting as a carrier and habitat for soil microbes, biochar can enhance microbial persistence, survival, and colonization in the rhizosphere, which are crucial for soil biogeochemical cycling, nutrient dynamics, and remediation of degraded soils. The physicochemical properties of biochar, including higher pore space, higher specific surface area and cation exchange capacity etc. create a conducive microenvironment for microbial proliferation and nutrient availability, thereby supporting plant growth. So, proper interactions between soil microbial communities (soil microbiome) and biochar and its consequences within the soil ecosystem need to be examined. This chapter examines properties of biochar and its effects on soil, characterization of biochar, interactions between biochar and soil microbiomes, and their function in promoting plant growth. It highlights that biochar-mediated soil microbiome improves soil functions, suppresses plant diseases, and contributes to sustainable agriculture and pollution mitigation.