The dynamic amplification factor (DAF) is a critical parameter in the design of highway bridges, which has attracted great interests from scholars and practitioners. Low-profile concrete bridges generally have shorter span length and lower self-weight, resulting in relatively greater dynamic responses under moving vehicles. This paper develops rigorous vehicle-bridge coupling models using commercial software package ABAQUS to simulate the dynamic interaction between specified vehicles and selected bridge superstructures, and DAF values for low-profile prestressed concrete bridges are further investigated. The 5-axle spatial vehicle is represented as a 3D mass-spring-damper system with 15 degrees of freedom and the bridge structure is modeled with sophisticated brick elements. The vehicle-induced dynamic responses of three different types of low-profile concrete bridges are investigated, including hollow slab beam bridge, T-girder bridge and spread slab beam bridge. DAF values inferred from displacement and strain responses are further compared among aforementioned three bridge types. Analysis results indicate that time-history dynamic responses of low-profile concrete bridges are influenced by the beam depth and section properties. Displacement-based DAF values are generally greater than strain-based DAF values, and the dynamic amplification effect of the spread slab beam bridge is more significant when compared with other two bridge systems, which should be handled with caution in the engineering practice.