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Orchids, belonging to the family Orchidaceae is considered to be a diverse plant species with its high aesthetic value. Gradually, this species are at a verge of extinction and being endangered. Nepal Himalaya has been reported to be a home for over 501 orchid species, many of which have been widely used in floriculture, medicine and food. Plant tissue culture technology has been used to reduce the depletion of this orchids by reducing the collecting pressure, using ex-situ conservation and reintroduction of endangered and extinct species back in nature. This chapter majorly focuses on the conservation of different forest based orchids used in floriculture industry addressing the important factors and role of plant tissue culture like in vitro propagation, symbiotic association, acclimatization and finally reintroduction. This chapter also highlights the recent advancements in asymbiotic seed germination, protocorm and vegetative regeneration technology of some important forest based orchids of Nepal.

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Tissue Culture Approaches for Conservation of Floriculturally Important Forest-Based Orchid Species

  • 章节

  • 发表日期：

    2026年

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  • Bijaya Pant,
  • Sushma Pandey,
  • Krissna Chand,
  • Bir bahadur Thapa

摘要

Orchids, belonging to the family Orchidaceae is considered to be a diverse plant species with its high aesthetic value. Gradually, this species are at a verge of extinction and being endangered. Nepal Himalaya has been reported to be a home for over 501 orchid species, many of which have been widely used in floriculture, medicine and food. Plant tissue culture technology has been used to reduce the depletion of this orchids by reducing the collecting pressure, using ex-situ conservation and reintroduction of endangered and extinct species back in nature. This chapter majorly focuses on the conservation of different forest based orchids used in floriculture industry addressing the important factors and role of plant tissue culture like in vitro propagation, symbiotic association, acclimatization and finally reintroduction. This chapter also highlights the recent advancements in asymbiotic seed germination, protocorm and vegetative regeneration technology of some important forest based orchids of Nepal.