Pooti (Hopea gregaria V. Slooten) is a species from the Dipterocarpaceae family with a limited distribution around the Tahura Nipa-Nipa area, Kendari, Southeast Sulawesi. This species can produce damar resin. However, the characteristics of H. gregaria resin are not widely known. This study aims to determine the characteristics and quality of H. gregaria sap resin. This research uses two methods, namely the visual test of SNI 2900 1.2012 and the laboratory quality test of SNI 2900 2.2013. Visual test results show clear yellow pooti resin and chunk size 3 × 3 cm. The impurity value of pooti damar is 0.44%, soft point value 75 °C, ash content value 0.01%, average value of turbidity in toluene 192.3 belonged to class II quality—the overall average was included in the first quality class. The visual and chemical-physical test of pooti resin shows that this damar meets the best quality requirements, class I based on SNI standards. These results also indicate that the resin quality of H. gregaria is equivalent to that of the best quality resins, such as resins from the Shorea javanica tree, and provides a great opportunity to utilize this pooti damar.