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The polymerase chain reaction (PCR) has had a profound impact on molecular biology since its development in the 1980s. Initially pioneered by Kary B. Mullis, PCR has revolutionized the field by allowing the targeted amplification, detection, quantification, and characterization of nucleic acids. PCR has become a standard tool in molecular biology laboratories, enabling various applications such as disease diagnosis, GMO detection, food authentication, mutagenesis, and genetic fingerprinting. Over the years, PCR has evolved from conventional endpoint PCR to quantitative real-time PCR and now to digital PCR, with each generation offering unique capabilities. The continuous development and modification of PCR techniques and chemicals have expanded the possibilities for research and diagnostics in the field of molecular biology.

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Evolution of PCR: From Classical PCR to Digital PCR

  • 章节

  • 发表日期：

    2026年

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  • Patrick Gürtler,
  • Sven Pecoraro

摘要

The polymerase chain reaction (PCR) has had a profound impact on molecular biology since its development in the 1980s. Initially pioneered by Kary B. Mullis, PCR has revolutionized the field by allowing the targeted amplification, detection, quantification, and characterization of nucleic acids. PCR has become a standard tool in molecular biology laboratories, enabling various applications such as disease diagnosis, GMO detection, food authentication, mutagenesis, and genetic fingerprinting. Over the years, PCR has evolved from conventional endpoint PCR to quantitative real-time PCR and now to digital PCR, with each generation offering unique capabilities. The continuous development and modification of PCR techniques and chemicals have expanded the possibilities for research and diagnostics in the field of molecular biology.