To limit climate change, the European Commission (EU) has set new objectives for GHG emissions for the transport sector, the EU Green Deal and the FitFor55 package. E-fuels, derived from low-carbon hydrogen and carbon dioxide captured or taken from unavoidable industrial point sources, are one of the sustainable solutions available to reduce the carbon footprint of the transport sector compared to conventional fuel. In parallel, an upcoming Euro 7 regulation has been proposed in order to limit the impact of the transport sector on pollutant emissions and air quality. A study was conducted using five gasoline Hybrid Electric Vehicles (HEV), from standards Euro 6d-temp and up, to assess the impact of conventional fuel and accurately simulated, surrogate advanced fuels with renewable hydrocarbons upon exhaust emissions. All the vehicles were equipped with aged TWC, and most of them with fresh GPF as well. Chassis dynamometer and on-road tests were performed to ensure a complete picture of emissions under both laboratory- and real-world conditions. State-of-the-art measurement equipment was used both on chassis dynamometer and on road, including FTIR giving access to insights into hydrocarbon and nitrogen compound speciation, as well as PN size distributions down to 10 nm in both engine-out and tailpipe positions via ELPI on chassis dynamometer. A detailed analysis of non-regulated and Euro 7 regulated pollutant emissions was performed for each of the vehicles. An assessment of the impact upon emission levels of the surrogate fuels compared to a reference conventional fuel was then made. Certain vehicles displayed specific sensitivities to certain fuels with regards to emissions of some pollutants, that were discussed with their respective manufacturers. Despite that, no major differences were observed between regular and e-fuels, showing the drop-in capability and the absence of impact on pollutant emissions of the low carbon e-fuels in addition to their reduced carbon footprint from a life cycle standpoint.