Electroporation, a technique that uses pulsed electric fields (PEF) to increase the permeability of cell membranes, is widely used in various biomedical applications, including cancer treatment, gene therapy, vaccine development, drug delivery, and the introduction of DNA or RNA into cells. It is also used in the food industry. The reliability and precision of the pulse generators that produce the PEF are crucial to the safety and effectiveness of this technology. However, emergencies such as power failures, device malfunctions, or operator errors can pose a significant risk, not only to the equipment but also to the patients undergoing electroporation. This chapter comprehensively analyzes existing emergency modes and methods for protecting pulse generators used in electroporation and highlights strategies for protecting devices and patients. The results emphasize the importance of incorporating failure protocols into pulse generators to minimize the risk of undesirable outcomes. The increasing use of electroporation technology emphasizes the need for continued innovation in emergency response strategies that ensure both device integrity and patient welfare in critical situations.