With the recent rapid growth of electroporation application and technology, an increased demand to identify treatment parameters best suited for each application has emerged. Experimentation on electroporative treatments often requires thorough testing across various pulsing parameters, necessitating streamlined methodology for reliable results. Here, we present a novel approach to automate the processes of electrode delivery to a cell monolayer and image resulting electroporative effects through the modification of a low-cost 3D printer into an automated electrode delivery system. This system enables efficient and accurate electrode placement within multi-well cell culture plates, alleviating the burden of manual electrode placement for the abundance of pulse parameter combinations. In comparison to the traditional method of electroporation using parallel plate electrodes in the form of cuvettes, the use of a non-uniform electric field applied to a monolayer allows for testing a range of electric field strengths in a single sample. This also eliminates the need for detachment of adherent cells prior to experimentation, avoiding additional stress and damage to cells. Furthermore, this method enables quick replacement of media providing control over the cell environment and facilitating testing of the effects of drugs, ions, chemicals, and other factors, expanding the capabilities of electroporation research. Coupled with automated image acquisition using specialized software, this method facilitates high-throughput experimentation for researchers to efficiently assess cell responses to pulsed electric field exposures and calculate LD50 electric field values. This automated approach enhances the reliability and efficiency of electroporation experimentation, contributing to advancements in the field of electroporation and pulsed electric field (PEF) ablation.