This study demonstrated that the printing orientation, with respect to the main CT reconstruction planes, influences significantly the measured Hounsfield Units (HU). Placing an object which print layers are perpendicular to the reconstructed CT plane causes the beam to average solid material and voids, resulting in lower HU values. Placing the objects which print layers are parallel to the plane minimizes this averaging effect, leading to higher HU values. Furthermore, the HU values remained consistent when measured in both the reconstructed axial and coronal views. Measured HU values were also found to be visually stable across both the Large and Narrow fields of view (FoV). This work provides practical guidelines for reliable CT scanning of 3D-printed phantoms, ensuring their validity as tissue replacements.