Lipid-based vesicular particles known as liposomes represent a special platform for tailored drug delivery. Thanks to their exceptional properties, including biocompatibility and biodegradability, liposomes are an ideal choice for drug delivery. Modification of the liposome surface improves the efficiency of drug delivery to the site of action in in vivo applications. The paper describes the integration of liposomes with nanofiber structures that improve the properties of liposomal carriers and their stability. These properties can be used in drug delivery systems. The aim of the experiment was to verify the incorporation of fluorescent dyes into liposomes and subsequently set up a system for drug delivery and diagnostic tools using a quantitative study and calibration curve. The results show the possibility of imaging incorporated liposomes in nanofibers using fluorescence microscopy and SEM microscopy. The amount of incorporated liposomes, their concentration, and incorporation into nanofibers were evaluated using a Zeta sizer and Nanodrop analysis. Fluorescent molecules such as FITC-dextran, which was encapsulated in liposomes, were detected using a fluorometric test.