Trust is the cornerstone of effective collaboration, shaping how individuals share knowledge, navigate uncertainty, and work toward shared goals. This chapter explores trust as a dynamic, multi-layered construct, spanning cognitive, affective, emotional, social, and psychological dimensions. Drawing on long-standing but timeless frameworks, it distinguishes between cognitive trust, built on competence and reliability, and affective trust, rooted in empathy and emotional connection. Focusing on Agile environments, particularly Scrum teams, the chapter examines how trust supports psychological safety, shared decision-making, and high performance in both co-located and distributed settings. It addresses common barriers to trust, including hierarchy, miscommunication, and past breaches, and presents enablers such as open dialogue, leadership vulnerability, and collective purpose. Trust is presented as something that must be built, maintained, and nurtured over time. Far from being a soft value, trust is shown to be a critical enabler of creativity, adaptability, and cohesion in complex, collaborative work.