This study investigates the microencapsulation of Ocimum sanctum L. (holy basil) essential oil using a high-shear homogenization method with Naterol®165V as the solid lipid matrix. The resulting solid lipid nanoparticles (SLNs) exhibited a mean particle size of 178.7 nm with a polydispersity index (PDI) of 0.34, as determined by dynamic light scattering (DLS). Scanning electron microscopy (SEM) confirmed the presence of spherical particles ranging from 200 to 400 nm. The SLN formulation demonstrated strong antioxidant activity, with a high encapsulation efficiency (EE) of 93.02 ± 1.53% and utilization efficiency (UE) of 97.35 ± 0.44%, indicating effective entrapment of active compounds. The SLN dispersion was successfully applied to cotton fabric via a soaking method. The total antioxidant activity retained on the fabric (Qtotal) was 61.18%, confirming efficient deposition. In a simulated aqueous environment, the release profile showed an initial burst followed by sustained release, with an estimated complete release time of approximately 530 min at a rate of 0.11% per minute. However, a significant decline in antioxidant activity was observed during storage, with a 57.21% reduction in Qtotal after 28 days at 45 ℃. These findings demonstrate the potential of Naterol-based SLNs in protecting volatile essential oils and maintaining their bioactivity on textile substrates, offering promising applications in the development of functional fabrics and cosmetotextiles.