The area devoted to the cultivation of industrial hemp (Cannabis sativa L.) in the European Union has increased in recent years, with France being the largest producer. This increase in hemp production is motivated by the production of natural fiber for use in textiles, paper and particularly in the construction industry, where insulating materials and hemp concrete are obtained. The design of the trial carried out in 2023 with 3 varieties of industrial hemp (Futura 75, Santhica 27 and Fibror 79) was 4 plots of 18 m2 in which the height of the crop was evaluated during its cycle plus its height, diameter, dry matter yield, percentage of long and short fiber in his harvest. The stem height and diameter of the Fibror 79 variety were higher than those of the Futura 75 and Santhica 27 varieties, respectively. Regarding the dry matter yield, Futura 75 and Fibror 79 showed similar data, with Santhica 27 being the variety with the lowest dry matter content. Finally, the short fiber content was higher in the Santhica 27 variety, with its long fiber content being lower than that of the other varieties. This trial shows a clear trend towards higher biomass and long fiber yields in varieties such as Futura 75 and Fibror 79, with the Santhica 27 variety being a usable variety due to its short fiber content.