This study explores the integration of citizen science and ecotourism to recover and conserve ecosystems in urban and rural areas, focusing on the Ilaló volcano in Quito, Ecuador. The research involved designing an Environmental Science Interpretation Trail through participatory methodologies, including community workshops, biodiversity monitoring using mobile applications like iNaturalist, and social mapping. Key findings include identifying 290 species along a 4,298-m transect, with data analyzed through diversity indices to inform conservation strategies. Community engagement was pivotal, with workshops fostering ownership and local capacity for environmental monitoring. The trail, mapped using tools like Wikiloc, features 13 points of interest, highlighting biodiversity hotspots, cultural landmarks, and areas affected by erosion and deforestation. Survey results indicated a strong interest in learning about local flora and fauna, the importance of tour guides, and the use of digital tools for navigation. Social media and community events were deemed effective for promoting the trail. The SWOT analysis highlighted strengths like a detailed trail guide and complementary agricultural activities, but weaknesses like an ageing community and limited tourism integration. The findings underscore the potential of citizen science and ecotourism to address urbanization challenges, promote conservation, and contribute to sustainable cities. The project demonstrates a replicable model for balancing urban development and natural resource preservation through community involvement and education.