Weaving technique has been used for many years to produce 3D textile structures and preforms, and many different methods have been developed. One of these methods is the weaving of conventional woven spacer fabrics with lancets. Woven spacer fabric structures consist of two different fabric layers where these fabric layers are connected to each other with pile yarns, leaving a certain space between them. Pile warp yarn interlaces through the top and bottom fabric and the distance between the layers can be adjusted using lancets with desired height. Lancets are mainly used across the entire fabric width without any missing for the production of conventional spacer fabrics. It is possible to arrange the lancets intermittently to a predefined width of chamber fabric. This may provide a flexibility for the production of spacer fabrics as side-closed structures in order to produce a chamber fabric with a targeted width. Spacer fabrics with partial chambers can be manufactured with less confection works for some pre-defined geometries and they can be used for the manufacturing of protective textiles and lightweight construction technology. This study aims to describe a new approach to the weaving of spacer fabrics with partial chambers with polyester multifilament yarns on a technical double rapier weaving machine. The setup of different warp yarn systems and the new lancet arrangement were presented and usability of this new approach for the manufacturing was discussed within the study. The applications of these newly developed spacer fabrics in the manufacturing of protective textiles was shown by means of textile-particle foam composites. Furthermore, the developed spacer fabrics with partial chambers were characterized according to their compressive resistance and energy absorption properties.