Eucalyptus is an economically significant plantation tree belonging to the Myrtaceae family and comprises more than 700 species worldwide. This species is valued for its rapid growth and commercial importance in timber, pulp, and bioenergy production, making it a widely cultivated resource. Eucalyptus is conventionally propagated by seeds; however, low germination rates, seed dormancy, and genetic variability affect plantation quality and productivity. To overcome these limitations, tissue culture has emerged as an effective solution for mass propagation in Eucalyptus. Micropropagation enables the mass production of genetically uniform, disease-free Eucalyptus seedlings with desirable traits. This chapter focuses on recent advancements in Eucalyptus micropropagation, specifically examining studies published between 2018 and 2024. The key factors influencing successful micropropagation, including the optimization of basal medium formulation and the application of plant growth regulators, will be synthesized and discussed.