Industry 5.0 represents a paradigm shift in manufacturing, emphasizing human-centricity, sustainability, and resilience alongside technological advancement. This study presents a targeted training intervention in the electronics industry designed to introduce and operationalize the pillars of Industry 5.0, specifically implementing in training. The intervention addressed internal company incentives to enhance human-centric practices for operators’ upskilling and wellbeing, as well as to embed new and procedures (such as green and resilient processes). Developed internally with support from training technology providers and monitored by a stakeholder responsible for overseeing Industry 5.0 adoption, the program combined theoretical instruction with experiential learning in a Learning Factory environment. Evaluation was conducted at both trainees’ and company levels – using self-assessed confidence linked to self-efficacy, and a pillar-based assessment framework. Results demonstrated improvements in perceived upskilling and Industry 5.0 indicators post-intervention. Additionally, statistical analysis revealed strong correlations between sustainability and the other two pillars. The findings highlight the role of structured, hands-on training as a foundational step toward the operationalization of Industry 5.0.