Within the expanding sustainability framework, the cultural dimension has increasingly gained relevance alongside environmental, economic, and social considerations. The inclusion of culture as another pillar of sustainability has posed a challenge that has been addressed from different perspectives when assessing its impact. Despite the consensus on the use of indicators, it is observed that cultural impact is primarily assessed during the service provision phase. This leaves the creation or end-of-life phases unexplored, necessitating a life cycle assessment perspective. To address the importance of assessing these phases, this study identifies the stakeholders in each life cycle phase (creation, provision, and end-of-life) through the analysis of a specific case of a cultural exhibition. The results show the importance of assessing cultural impact throughout the entire life cycle and the challenges associated with its measurement. The effectiveness of using participatory methodologies is identified, as is the need to expand the Life Cycle Sustainability Assessment (LCSA) framework to fully integrate culture. Thus, this work contributes to the development of more comprehensive sustainability assessment tools applicable to cultural services and products under a holistic life cycle approach.