This study investigates brand attention during non-skippable in-stream advertisements (ads), with a focus on potential gender differences. While previous research highlights the importance of brand attention as a key outcome of advertising efforts, the duration of brand attention in this context remains underexplored. We conducted an experiment with 77 participants (41 females) in a controlled laboratory setting at a Brazilian university, measuring gaze duration across five Areas of Interest (AOIs) representing brand elements. Our findings revealed that total brand attention averaged 2.81 s for females and 3.09 s for males, primarily concentrated in the biggest AOIs. Our hypothesis positing no significant gender differences in brand attention was partially supported. Assuming a liberal approach, we can establish equivalent means across genders, suggesting similar visual behavior across both groups. With our findings, we emphasize the need for advertisers to maximize exposure to brand features throughout the advertisement. These results contribute to a deeper understanding of brand attention dynamics in streaming video and highlight practical implications for advertising strategies.