Fungal choroiditis is a vision-threatening inflammatory condition affecting the choroid, often resulting from hematogenous dissemination during systemic fungal infections or local spread from adjacent ocular structures. Candida and Aspergillus species are the most commonly implicated pathogens. Clinical manifestations include blurred vision, floaters, and photophobia, necessitating a thorough ophthalmic examination for timely diagnosis. Given the limitations of traditional culture-based methods, a multimodal diagnostic approach incorporating PCR-based assays, galactomannan, beta-D-glucan testing, and advanced imaging is critical for early detection. Imaging modalities such as fluorescein angiography, spectral domain optical coherence tomography, and fundus autofluorescence aid in disease characterization and treatment monitoring. Management involves systemic and intravitreal antifungal therapy, tailored to disease severity. Due to its complexity, fungal choroiditis requires multidisciplinary collaboration among ophthalmologists, infectious disease specialists, and critical care teams to optimize outcomes and prevent complications. This chapter explores key diagnostic, prognostic, and therapeutic challenges in managing this condition.