Virtualization has transformed into an essential tool for systems administration, allowing the evaluation and comparison of distinct operating systems in controlled and isolated environments to optimize resource usage and reduce costs. This study compares the performance of FTP, Web, and DNS services in two Linux-based distributions, Tiny Core and Debian, employing an experimental methodology. Both systems were virtualized in VirtualBox, under identical hardware conditions to ensure the impartiality of the results. Performance metrics included CPU, RAM, and disk space usage, as well as service installation and response times. Although Debian presented advantages in installation time and response to requests, Tiny Core showed its efficiency in the use of resources and consumed less disk space. However, Tiny Core lacks configuration persistence after reboot, making it suitable for long-term environments, whereas Debian is better suited for stable environments. The choice between the two distributions depends on the user’s specific requirements.