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The present research describes the molecular identification of Ecuadorian species of the Araceae family with commercial interest using DNA barcoding through specimen sampling, molecular fingerprinting, and phylogenetic and bioinformatic analysis methodologies. Twenty specimens of the genera Alocasia (1), Anthurium (10), Amorphophallus (1), Monstera (5), and Philodendron (3) from EquaFlor-A greenhouses in Azuay and Zamora Chinchipe were analyzed. For molecular fingerprinting, the matK and rpoC1 markers were used, and their sequencing products were compared with the GenBank database through the BOLDSystems and BLAST search engines. This analysis allows the molecular identification of 85% of the species. The remaining 15% were identified morphologically with reference specimens from certified herbaria. The phylogenetic analysis evidenced the evolutionary relationship between Monstera and Anthurium, as well as the proximity between Alocasia, Amorphophallus, and Philodendron within the subfamily Aroideae. These findings highlight the effectiveness of DNA barcoding as a complementary tool for the taxonomic analysis of the Araceae family.

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DNA Barcoding of Ecuadorian Araceae Species of Commercial Interest

  • 会议论文

  • 发表日期：

    2026年

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  • María José Montesdeoca,
  • Marco Cerna,
  • Mario Portilla

摘要

The present research describes the molecular identification of Ecuadorian species of the Araceae family with commercial interest using DNA barcoding through specimen sampling, molecular fingerprinting, and phylogenetic and bioinformatic analysis methodologies. Twenty specimens of the genera Alocasia (1), Anthurium (10), Amorphophallus (1), Monstera (5), and Philodendron (3) from EquaFlor-A greenhouses in Azuay and Zamora Chinchipe were analyzed. For molecular fingerprinting, the matK and rpoC1 markers were used, and their sequencing products were compared with the GenBank database through the BOLDSystems and BLAST search engines. This analysis allows the molecular identification of 85% of the species. The remaining 15% were identified morphologically with reference specimens from certified herbaria. The phylogenetic analysis evidenced the evolutionary relationship between Monstera and Anthurium, as well as the proximity between Alocasia, Amorphophallus, and Philodendron within the subfamily Aroideae. These findings highlight the effectiveness of DNA barcoding as a complementary tool for the taxonomic analysis of the Araceae family.