One of the challenges entailed in today’s agrifood supply is related to their environmental impacts, related in particular to climate change, freshwater depletion, land use change and dispersion of pollutants in the environment. Looking specifically at the wine sector, producers are trying to pursue sustainable production practices, as product sustainability is becoming more and more relevant to consumers. This work presents the analysis of the Carbon Footprint (CF) of two types of wine produced in a company from Southern Italy, Ettamiano and Tanaia, based on a life-cycle approach. Results are coherent with previous studies, highlighting the good practices adopted by the company analyzed, and showing the hotspots of wine production (the packaging and winery phases). Consequently, some guidelines and recommendations for improving the CF of wine production are provided. This study contributes to building a base for future improvements of the environmental impact of wine supply chains, based on Circular Economy strategies.