This study aimed to diagnose active compounds in the Mirabilis jalapa plant root powder and assess the effect of gradually increasing plant supplemental levels on carcass characteristics and growth performance. The experimental design included collecting plant roots and drying them, then extracting them by the continuous extraction method (Soxhlet), using organic solvent hexane, and then detecting biocompounds by GC-Mass. The experiment used 240 chicks divided into (4) treatments and continued until the experiment. The study duration was (42) days, and the study treatments were control treatment (no addition), treating the M. jalapa with a dose of 0.5, 1 and 1.5 g/kg feed. The results of GC-Mass showed the presence of a variety of pharmacological activities in M. jalapa, containing seven fatty acids palmitic acid, arachidonic acid, margaric acid, stearic acid, lauric acid, tridecylic acid, phytopositosterol. The results of the statistical analysis showed there is an improvement in growth performance and that there were significant differences in live body weight at the sixth week in all treatments compared to the control, especially at the dose of 1.5 gm/kg, which amounted to 3655 g. The weight gain amounted to 1218 g and the feed conversion ratio was 0.82. Body parts showed significant superiority for the weight of the chest, thighs, back, neck and wings at the 1.5 g/kg dose and amounted to 1064, 980.8, 258.3, 127.5 and 270.8 g, respectively. It can be concluded from the current study that the roots of the plant are rich in active substances that can be used sustainably as an alternative to other feed additives that have harmful effects on human and animal health.