Achieving optimal glycemic control in critically ill patients remains a major challenge in intensive care units (ICUs), particularly in resource-limited environments. We present to you a proof of concept of a preliminary validation of a real-time blood sampling system intended to enable continuous glucose monitoring (CGM) in intensive care units. The prototype integrates an automated micro-controlled circuit for periodic blood withdrawal and return, coupled with a real-time glucose sensing module operating within a closed heparinized loop. A plasma-mimicking fluid was used to assess flow stability, sampling accuracy, and mechanical reliability under controlled laboratory conditions. Early tests showed stable hydraulic behavior, reproducible sampling cycles, and reliable glucose readings within physiological ranges. The system’s modular and low-cost design allows for easy integration into standard ICU workflows and represents a promising alternative to conventional CGM technologies. Further studies will focus on in vivo validation and optimization for clinical application in low- and middle-income healthcare settings.