Pharmacogenetics (PGx) testing is essential for personalised medicine, as it enables tailored treatments and reduces the risk of adverse drug reactions based on individuals’ genetic profiles. This study aimed to compare the educational exposure, knowledge, and willingness to undergo PGx testing between medical and pharmacy students in Malaysia. We conducted an online survey using a validated questionnaire, involving 405 respondents. The results indicated that pharmacy students had significantly more training in PGx (56.5% vs. 25.5%, p < 0.001) and better awareness of PGx testing (77.4% vs. 41.4%, p < 0.001). Both groups showed a similar willingness to engage in PGx research (71.8% among pharmacy students vs. 68.8% among medical students, p = 0.521) and to attend related courses (87.5% vs. 83.4%, p = 0.252). Overall, pharmacy students demonstrated higher levels of educational exposure, greater knowledge and positive attitudes towards PGx. These findings highlight the importance of integrating PGx education into medical training to enhance healthcare outcomes.