As cities continue to dominate the actual global stage, producing the majority of the world’s Gross Product, housing almost 60% of the global population, and generating most of the world’s Greenhouse Gas Emissions, they have become the centre of attention in the transition towards sustainability. In response to the escalating climate and environmental crises, the European Union launched in 2019 the European Green Deal, a comprehensive framework of strategies designed to drive sustainable transformation across all member states. This work focuses on the implementation of one of the key frameworks of the Green Deal, the European 2030 Biodiversity Strategy, examining the latest awarded European Green Capitals proposals through an in-depth analysis of three of the four winners since the announcement of the Biodiversity Strategy (Grenoble, Valencia, and Vilnius). This paper seeks to identify which goals within the pillars have seen progress in the urban environments, in which measure they have been implemented, their distribution, and reasoning behind their lag or excess of implementation, and how close, our primary green city referents, find themselves to achieving the goals and targets of the EU 2030 Biodiversity Strategy.