Domestic donkeys have interacted with human civilizations for thousands of years as pack and transportation animals, and indeed they still are, particularly in the driest and poorest countries of the world. Additionally, the beneficial effects of donkey milk for human health have been known and documented for over 2000 years. This knowledge has laid the foundations for the current growth of commercial production systems, primarily aimed at producing milk for children and elderly adults who are allergic to cow milk proteins (CMA). Additionally, the peptides from donkey milk are used in the human cosmetic industry. As with any milk production system, donkey milk production requires a focus on reproduction. These farms are mostly semi-intensive and based on pasture natural breeding. These conditions align with donkeys’ natural behaviors, reducing the need for intensive human handling and thereby decreasing stress, resulting in positive and cost-effective outcomes for both the animals and the system. However, considering future genetic improvement programs and the preservation of lines and breeds, assisted reproduction techniques such as embryo transfer, embryo cryopreservation, semen cryopreservation, and in vitro embryo production will be necessary. While these techniques are currently being developed for donkeys, they remain in the experimental stages.