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The nanopreservation is an emerging technology for reducing postharvest diseases of red bell pepper fruit that has gained interest in recent years. The aim of this work was to present the factors that affect the extraction process of nance (Byrsonima crassifolia L.) to scale up the nanoprecipitation method used for chitosan nanoparticles (CSNPs) elaboration and evaluate the effect of nanocoatings based-chitosan-nance extracts (NE) on Alternaria alternata in vitro and on the incidence and severity on red bell pepper fruit. The color measured in nance leaves showed similar value. The change in drying temperature (40, 50, and 60 °C) did not show significant differences in moisture percentage and dry matter yield. The highest yield for extraction was for the dry matter/ethanol ratio of 1:5. The particle size distribution and Zeta potential were not affected by the scaling-up process. Uni, bi and trimodal particle size distributions were observed with particles size larger than 200 nm. For the evaluation of the in vitro growth of A. alternata, percentage inhibition ranged from 23.60 to 100%. The lowest incidence and severity of A. alternata was for the nance extract loaded-chitosan nanoparticles (NECSNPs) coating (0.5%) with values of 70% and 8.10 ± 1.2 mm, respectively. The infection was delayed, when the red bell peppers were coated with NECSNPs after 10 days of storage at room temperature.

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Study of Variables of Scaling Up the Nance Extraction Process and Chitosan Nano Encapsulation, and Its Effect In Vitro and on Black Rot Growth of Red Bell Peppers

  • 章节

  • 发表日期：

    2026年

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  • Claudia Natalia Hernández-López,
  • Zormy Nacary Correa-Pacheco,
  • Laura Leticia Barrera-Necha,
  • Silvia Bautista-Baños,
  • Mónica Hernández-López,
  • Carlos López-González

摘要

The nanopreservation is an emerging technology for reducing postharvest diseases of red bell pepper fruit that has gained interest in recent years. The aim of this work was to present the factors that affect the extraction process of nance (Byrsonima crassifolia L.) to scale up the nanoprecipitation method used for chitosan nanoparticles (CSNPs) elaboration and evaluate the effect of nanocoatings based-chitosan-nance extracts (NE) on Alternaria alternata in vitro and on the incidence and severity on red bell pepper fruit. The color measured in nance leaves showed similar value. The change in drying temperature (40, 50, and 60 °C) did not show significant differences in moisture percentage and dry matter yield. The highest yield for extraction was for the dry matter/ethanol ratio of 1:5. The particle size distribution and Zeta potential were not affected by the scaling-up process. Uni, bi and trimodal particle size distributions were observed with particles size larger than 200 nm. For the evaluation of the in vitro growth of A. alternata, percentage inhibition ranged from 23.60 to 100%. The lowest incidence and severity of A. alternata was for the nance extract loaded-chitosan nanoparticles (NECSNPs) coating (0.5%) with values of 70% and 8.10 ± 1.2 mm, respectively. The infection was delayed, when the red bell peppers were coated with NECSNPs after 10 days of storage at room temperature.