It takes a lot of skill to identify human trafficking victims because, for a variety of reasons, the victims are reluctant to report the crime. Obtaining information from victims also requires a variety of methods and approaches. In order to comprehend human trafficking, the chapter outlines the forensic evaluations and procedures that must be adhered to. It highlighted several techniques for identifying victims in a variety of contexts, such as hospitals, emergency services, and other circumstances. Forensic medical evaluations in human trafficking cases are crucial and serve multiple purposes, including assessing the case, addressing medical needs, collecting public health data, and supporting the victim. Victims of human trafficking may experience psychiatric issues, making screening for these conditions essential. Evaluations are described in detail. The chapter also mentioned the requirements for the investigation of human trafficking. Though a lot of progress has happened in this area, there is a long way to go for forensic investigations into human trafficking, as it always presents challenges to current techniques and methods due to rapid technological development and its use in recruitment and trafficking processes. It is essential to continually upgrade the skills of investigative agencies.