Cephalopods including squids, cuttlefish and octopus are ecologically and commercially important molluscans. In this study, samples of Long Barrel squid (Uroteuthis singhalensis) from selected fish landing centres of southeast and southwest coasts of India were subjected to landmark based morphometric studies and multivariate analysis. Taxonomic confirmation of the species was attempted using DNA barcoding. Distinct variations in morphometric characters were noted among four populations of U. singhalensis. Discriminating trend in body, beak, statolith and gladius morphometrics were observed among populations. The results suggest the possibility of the influence of different coastal environments and genetic factors in bringing out the observed variations in morphological characters among the different populations of squids.