To ensure the high competitiveness of cotton products in Uzbekistan, modern approaches are being implemented to enhance both the yield and quality of cotton fiber. One such innovation is the gene knockout technology, developed by Uzbek scientists and patented in several cotton-producing countries. This advancement enabled the creation of unique genetically modified cotton varieties of the Porlock (P) series, which exhibit improved characteristics in terms of cultivation, vegetation, and fiber quality. These new genetically modified cotton varieties possess distinctive structural and volumetric properties compared to zoned varieties, necessitating adjustments in chemical finishing technologies. Technological parameters for the preparation processes have been developed with consideration of the structural and sorption characteristics of textile materials derived from Porlock cotton fiber. Cost-effective technological regimes and bath compositions for dyeing and printing preparation of textile materials (yarn and fabric) have been proposed, tailored to the structural features of these new cotton varieties. The preparation mode for dyeing cotton yarn of the P-2 and P-4 selection varieties has been successfully implemented at LLC “OSBORN TEXTILE”. As a result, high whiteness, enhanced capillarity, and improved physical and mechanical properties were achieved, aligned with the structural characteristics of the cotton yarn. Specifically, the P-2 variety, characterized by a denser structure, requiresrelatively high concentrations of alkaline agents (up to 10%) and lower concentrations of hydrogen peroxide (up to 17%) during preparation. Incontrast, the P-4 variety, with its higher volumetric properties, requires approximately 20% less alkaline agent.