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A novel fluorescence detection system designed to enhance Point-of-Care (PoC) real-time PCR instruments is presented. It uses a modular array of readout units coordinated via a custom communication protocol. An advanced image processing pipeline corrects perspective distortions to create accurate composite fluorescence maps. The realized prototype improves flexibility, spatial resolution, spectral range, and optical uniformity. Real-time PCR assays validate its quantitative performance, demonstrating its broad applicability.

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A Multi-camera Fluorescence Detector for Enhancing Point-of-Care Instruments for Biomolecular Analyses

  • 会议论文

  • 发表日期：

    2026年

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  • Walter Carli,
  • Antonella Buquicchio,
  • Alessio Nicola,
  • Marco Carminati,
  • Marco A. Bianchessi

摘要

A novel fluorescence detection system designed to enhance Point-of-Care (PoC) real-time PCR instruments is presented. It uses a modular array of readout units coordinated via a custom communication protocol. An advanced image processing pipeline corrects perspective distortions to create accurate composite fluorescence maps. The realized prototype improves flexibility, spatial resolution, spectral range, and optical uniformity. Real-time PCR assays validate its quantitative performance, demonstrating its broad applicability.