Hybrid timber-masonry structural systems are commonly found in traditional and historical buildings, representing a significant portion of the global built heritage. Traditional timber-framed masonry structures have historically demonstrated good seismic performance in earthquake-prone regions. While this behavior is often studied and validated through experimental analyses, many valuable typologies remain unexplored. Experimental data are essential for the reliable analysis, preservation and rehabilitation of traditional timber-masonry structures. This study presents an experimental investigation aimed at assessing the seismic behavior of a typical timber-masonry load-bearing panel found in historical buildings in Valparaíso, Chile (a city listed in the UNESCO World Heritage Site since 2003). The frame is configured by studs, plates and diagonals connected by traditional carpentry joints, and filled with unreinforced brick masonry panels using lime mortar. The experimental campaign involved cyclic lateral load tests conducted on wall panels with and without infill. Specimens were constructed at 1:3 scale using both manual and robotic machining techniques. The objectives of this study are to determine the lateral load-bearing capacity of the wall and to evaluate the contribution of the unreinforced brick masonry infill in terms of strength, stiffness, ductility, and energy dissipation. The test results indicated that the infill can significantly enhance the wall’s stiffness, ductility, and ultimate load-bearing capacity. Furthermore, typical failure modes were identified, providing valuable insights for the design of preventive structural reinforcement strategies to mitigate damage in future earthquakes.