Agile software development promotes adaptability, collaboration, and continuous delivery. However, implementing Agile principles remains a challenge for many organizations due to complex organizational, cultural, and interpersonal factors. Building on a previous empirical study that identified the most difficult Agile principles to apply and their underlying causes, this paper extends the investigation by analyzing the barriers that practitioners perceive as limiting Agile adoption in Romanian IT industry exploring human factors compared to computer science world wide industry. Using a mixed-method approach and Straussian Grounded Theory, we developed a concept model that links causes, barriers, and mitigation strategies. The results indicate that barriers often emerge from unresolved causes and can reinforce them over time, leading to persistent implementation difficulties. The study identifies five major categories of barriers and highlights that successful mitigation requires both process-level improvements and cultural transformation. The findings provide actionable insights for practitioners and contribute to a more nuanced understanding of Agile implementation challenges.