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To better understand the impact of mixed cephalosporin antibiotics on the denitrification system, this experiment used cephalexin (CFX) and ceftizoxime (CEX) as target pollutants. A sequential batch reactor (SBR) was employed to investigate the effects of a mixed concentration of CFX + CEX = 5 + 5 mg/L on denitrification performance, antibiotic degradation pathways, and microbial community structure. Results indicate that after adding the antibiotic mixture, nitrate nitrogen removal efficiency gradually decreased, accompanied by NO2− accumulation. This accumulation can serve as an electron acceptor for Anammox studies, addressing the issue of insufficient NO2––N supply. This study proposed three novel degradation pathways distinct from previous research. Additionally, potential antibiotic-degrading bacteria, Exiguobacterium and Stenotrophobacter, were identified. This study presents an economical and environmentally friendly wastewater treatment method, providing theoretical guidance for advancements in urban environmental pollution control.

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Impact of Mixed Cefalexin and Ceftizoxime Stress on Denitrification Performance and Microbial Communities

  • 会议论文

  • 发表日期：

    2026年

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  • Meiling Ma,
  • Haoyuan Ma,
  • Ziyun Yan,
  • Yonghui Zhang,
  • Yongzhi Chen

摘要

To better understand the impact of mixed cephalosporin antibiotics on the denitrification system, this experiment used cephalexin (CFX) and ceftizoxime (CEX) as target pollutants. A sequential batch reactor (SBR) was employed to investigate the effects of a mixed concentration of CFX + CEX = 5 + 5 mg/L on denitrification performance, antibiotic degradation pathways, and microbial community structure. Results indicate that after adding the antibiotic mixture, nitrate nitrogen removal efficiency gradually decreased, accompanied by NO2− accumulation. This accumulation can serve as an electron acceptor for Anammox studies, addressing the issue of insufficient NO2––N supply. This study proposed three novel degradation pathways distinct from previous research. Additionally, potential antibiotic-degrading bacteria, Exiguobacterium and Stenotrophobacter, were identified. This study presents an economical and environmentally friendly wastewater treatment method, providing theoretical guidance for advancements in urban environmental pollution control.