This paper presents the development of advanced electromagnetic (EM) sensor utilizing novel metamaterial-inspired modified dual-SRR based resonators for non-invasive detection of glucose levels in biological samples. The sensor resonator employs a dual-SRR along with two SRRs to detect glucose concentration in physiological solution mimicking human blood. The best-suited optimized dimensions for the proposed sensor resonator structure is carried out using the Genetic-Algorithm within the framework of the CST Microwave-Studio. Leveraging frequency-shifting properties this sensor resonator prototyped on FR-4 substrate, detects a significant shift of 1 GHz in resonant frequency for 100 mg/dL and 200 mg/dL glucose-DI water physiological solution. This proposed sensor is compact, robust, inexpensive and sensitive makes it a good alternative in non-invasive blood glucose detection in biomedical industries.