The global health community is greatly concerned about the emergence and spread of antibiotic resistance as well as the evolution of new strains of disease-causing agents. Medicinal plants that are commonly used have the potential to be an excellent source of drugs to address these issues; one such plant is Caryophyllum aromaticus This study aims to assess the synergistic effect of Caryophyllum aromaticus in combination with cefotaxime against Pseudomonas aeruginosa Specifically, the objectives included determining minimum inhibitory concentrations (MICs) using the micro-dilution broth method, and evaluate the antibacterial activity before and after adding of clove. The micro-dilution broth method was used to investigate the antimicrobial susceptibility of Pseudomonas aeruginosa isolates in order to determine the minimum inhibitory concentrations (MICs) for cefotaxime alone and in combination with clove. Checkerboard experiments were performed to evaluate synergy. To guarantee proper evaluation of bacterial growth and susceptibility patterns, cultured methods were used. According to the study, there was a considerable boost in the antibacterial activity against Pseudomonas aeruginosa when Caryophyllum aromaticus and cefotaxime were combined. According to microdilution broth experiments, the MIC values for cefotaxime alone against Pseudomonas aeruginosa ranged from 2 to 8 mg/L, with certain samples exhibiting total resistance. But after Pseudomonas aeruginosa was exposed to extract from C. aromaticus, the MIC values for every sample fell between 12.5 and 0.78 mg/ml. Cefotaxime's MIC values ranged from 0.5 to 8 mg/L for intermediate samples and the study shows an effective enhancement of cefotaxime MIC in MDR samples indicating that the antibiotic's efficacy was greatly increased when combined with C. aromaticus extract. According to the study's findings, C. aromaticus and cefotaxime work better together to inhibit Pseudomonas aeruginosa, as seen by lower minimum inhibitory concentrations. For Pseudomonas aeruginosa (intermediate), the minimum inhibitory concentration (MIC) of C. aromaticus extract is 2–8 mg/L. The antibiotic's minimum inhibitory concentration (MIC) dropped to a range of 0.5–8 mg/L after addition of cefotaxime. These findings demonstrate the potential of C. aromaticus as an adjunctive treatment for Pseudomonas aeruginosa infections resistant to antibiotics.