In recent years, a focus on the involvement of neuroinflammation in psychiatric disorders including Obsessive Compulsive Disorder (OCD) has emerged. Studies described ways in which neuroinflammation, caused by immune system imbalance or other factors, could play a role in the onset and continuation of OCD symptoms. Toll like receptor 4 (TLR4) is part of pattern recognition receptors (PRR) aided in pathogen recognition by phagocytic cells through Lipopolysaccharide (LPS). Polymorphisms in such receptors may leads to overactivation and uncontrolled cytokine secretion. Thus this study aimed to test for the presence of SNPs in TLR4 and determine its association to OCD in Jordanian population. 56 OCD patients and 56 control participants, matched by age and sex, made up the case–control study’s participant the group. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR–RFLP) was used to assess TLR4 polymorphisms. The Nco1 enzyme was used to digest the TLR4 (rs4986790) SNP whereas, Hinf1 enzyme was used for TlR4 (rs4986791) SNP. Sanger sequencing was conducted for 20 samples of each SNP to confirm RFLP-PCR results. Results revealed the absence of both tested TLR-4 polymorphisms (rs4986790 and rs4986791) in all investigated OCD and control subjects. Sanger sequences confirmed the RFLP-PCR results. Although TLR4 are immunomodulatory and are implicated in neuroinflammatory processes, it appears that these particular genetic variants are not associated with OCD.