This chapter uses a systems thinking approach to conceptualise the Ecosystem of Trust in Science as a dynamic and evolving system shaped by changing research and innovation environments. Contemporary science is increasingly internationalised, oriented toward addressing grand societal challenges, and driven by rapid technological advances. As a result, the Ecosystem of Trust has expanded beyond individual researchers to include a diverse and distributed set of actors. The chapter maps Stewards of Trust and key stakeholders within this ecosystem, taking into account diversity within both Stewards of Trust and the publics whose trust is at stake. While existing research has examined mediators of trust between science and society, it has largely overlooked the relational and systemic dynamics through which trust is constructed, negotiated, enhanced or reduced. This chapter addresses this gap by providing a framework that captures interdependencies among Stewards of Trust within a complex and evolving Ecosystem of Trust.