This study investigates the integration of aramid nanofibers (ANFs) into the poly (ethylene glycol) diacrylate (PEGDA) biopolymer to enhance its mechanical properties and promote cell adhesion for application as a scaffold in tissue engineering. The research commenced with the synthesis of ANFs at the nanoscale, followed by the utilization of digital light processing 3D printing technology to fabricate the scaffolds. The experimental design aimed to determine the most effective printing parameters for producing scaffolds with high strength, as indicated by Young’s modulus values. Various factors were manipulated, including PEGDA concentrations of 30%, 40%, and 50% by weight, resin to ANF ratios of 10:0, 9:1, and 8:2, and curing times of 60, 70, and 80 s. The results indicated that the scaffold with an 8:2 resin to ANF composition exhibited non-toxic behavior, with a cell viability loss of less than 30% in the MTT assay. Notably, the highest printable resin formulation with a 7:3 ANF ratio resulted in a total cell viability of 106.63% and enhanced cell proliferation. The findings suggest that PEGDA/ANF scaffolds possess promising mechanical and biocompatibility characteristics for use in 3D tissue engineering applications.