European cultural routes serve not only as vital conduits for preserving shared cultural heritage, but also as strategic instruments for advancing territorial cohesion and sustainable regional development. The value of these transnational networks lies in their complex integration of architectural remains, historical landscapes, and living traditions. Interpreting this value requires a multidimensional and spatially recognition approach that moves beyond static heritage typologies to embrace the dynamic relationships between material cultural assets and their surrounding environments. This study adopts a landscape-based perspective to investigate the Via Francigena, one of Europe’s most famous and oldest pilgrimage routes. Focusing on the Italian section, it identifies two primary material-based interpretive dimensions of the route: the physical architectural remains, and the cultural landscapes associated with intangible assets such as local knowledge, memory practices, and pilgrimage traditions. Building upon Italian regional planning practices, the research then explores how landscape inventory methods have been employed to classify and manage cultural routes not by administrative boundaries but through landscape units, enabling segmental governance and integrated conservation strategies. Furthermore, the study highlights how slow mobility, including walking and cycling, serves as an embodied and immersive way to experience the spatial and cultural narratives embedded in the route, thereby raising public awareness of its heritage value.