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Oil spillsOil spill represent a significant threat to marine ecosystems, yet their immunotoxic effects on economically important invertebrates like sea cucumbersSea cucumber remain poorly understood. This study investigated the impacts of Oman crude oil water-accommodated fractions (WAFs) on the immune function of sea cucumberSea cucumber (Apostichopus japonicus) coelomocytes using high-throughput RNA sequencing. Sea cucumbersSea cucumber were exposed to WAFs exposure solution at the nominal concentrations of 25 and 75% (v/v) for 7 d. Transcriptome analysis revealed 4911 and 5405 differentially expressed genes (DEGs) in the 25 and 75% (v/v) WAF groups, respectively, compared to the control. Gene Ontology (GO) enrichment analysis indicated that these DEGs were prominently involved in biological adhesion, immune system processes, and antioxidant activity. KEGG pathway analysis revealed significant enrichment in immune-related pathways, including NOD-like receptor signaling, Toll-like receptor signaling, and cytosolic DNA-sensing pathways. Notably, the number of immune-related pathways decreased with increasing WAF concentration (6 pathways at 25% (v/v); 4 pathways at 75% (v/v)), suggesting a low-concentration stimulation and high-concentration inhibition pattern of immunotoxicityImmunotoxicity. These findings could provide novel insights into the molecular mechanisms underlying oil pollution-induced immunotoxicityImmunotoxicity in marine invertebrates and establish a foundation for developing biomarkers for ecological risk assessment of oil contamination in marine environments.

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Transcriptomic Analysis Reveals Immunotoxic Effects of Oman Crude Oil Water-Accommodated Fractions on Sea Cucumber (Apostichopus Japonicus) Coelomocytes

  • 会议论文

  • 发表日期：

    2026年

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  • Xishan Li,
  • Xin Li,
  • Feng Guo,
  • Fu-en Chen,
  • Yongqi Liang,
  • Haonan Wang,
  • Deqi Xiong

摘要

Oil spillsOil spill represent a significant threat to marine ecosystems, yet their immunotoxic effects on economically important invertebrates like sea cucumbersSea cucumber remain poorly understood. This study investigated the impacts of Oman crude oil water-accommodated fractions (WAFs) on the immune function of sea cucumberSea cucumber (Apostichopus japonicus) coelomocytes using high-throughput RNA sequencing. Sea cucumbersSea cucumber were exposed to WAFs exposure solution at the nominal concentrations of 25 and 75% (v/v) for 7 d. Transcriptome analysis revealed 4911 and 5405 differentially expressed genes (DEGs) in the 25 and 75% (v/v) WAF groups, respectively, compared to the control. Gene Ontology (GO) enrichment analysis indicated that these DEGs were prominently involved in biological adhesion, immune system processes, and antioxidant activity. KEGG pathway analysis revealed significant enrichment in immune-related pathways, including NOD-like receptor signaling, Toll-like receptor signaling, and cytosolic DNA-sensing pathways. Notably, the number of immune-related pathways decreased with increasing WAF concentration (6 pathways at 25% (v/v); 4 pathways at 75% (v/v)), suggesting a low-concentration stimulation and high-concentration inhibition pattern of immunotoxicityImmunotoxicity. These findings could provide novel insights into the molecular mechanisms underlying oil pollution-induced immunotoxicityImmunotoxicity in marine invertebrates and establish a foundation for developing biomarkers for ecological risk assessment of oil contamination in marine environments.