As the demand for seafood is continuously increasing worldwide, traditional fishmeal- and fish oil-based diets poses significant environmental consequences. Therefore, there is an urgent need for sustainable alternatives. This chapter focuses on the revolution in aquaculture nutrition with the array of omics technologies such as transcriptomics, proteomics, metabolomics, and microbiomics. Studies carried out with advanced omics technologies enable the analysis of biological processes at the molecular level, resulting in an understanding of the needs of fish in terms of dietary requirements and their responses to different feed formulations. These innovations will help to produce feeds that optimize fish growth, health, and immune function with minimum ecological impact. Some principal applications of omics in aquaculture include the formulation of species-specific feeds, nutrigenomics for gene-diet interactions, and microbiomics for gut health. Furthermore, integrative multi-omics analysis provides a fine-scale understanding of fish physiology for precision nutrition and improved feed efficiency, although challanges exist, such as high costs and complexities associated with integrating data. The future potential of omics will lie in using artificial intelligence in real-time diet optimization by analyzing the omics data. This area of research promises to support a more sustainable aquaculture industry with minimal environmental damage as the research in omics advances.