Fuel stations are facilities that go beyond supplying fuel to vehicles, also offering a range of complementary services such as car washing and vehicle maintenance. These activities generate various types of waste, including used oils, solvents, sludge from oil/water separators, absorbent materials, filters, protective clothing, and cleaning rags. Many of these wastes may be contaminated with Persistent Organic Pollutants (POPs), chemical substances that exhibit high environmental persistence, potential for bioaccumulation in living organisms, and harmful effects on human health and ecosystems. Due to their chemical stability, POPs can remain in the environment for extended periods and disperse globally through air and water currents as well as species migration. This study aims to identify and quantify the concentration of POPs in the waste generated at fuel stations, comparing the results with the legal limits established by Regulation (EU) 2019/1021 of 20th June. The research also highlights the importance of proper management practices for contaminated waste, ensuring the destruction or irreversible transformation of POPs. The proper segregation and disposal of waste on-site are essential to prevent environmental contamination and protect public health, reinforcing fuel stations’ commitment to sustainability and compliance with environmental regulations.